Concept:Contractile vacuoles in Amoeba regulate water balance by expelling excess water that enters the cell.
Their activity depends on the osmotic difference between the cytoplasm and the surrounding medium.
Explanation:A salt solution is hypertonic compared to the cytoplasm of the Amoeba.
Therefore, water tends to move out of the cell by osmosis.
As a result, less excess water accumulates inside the Amoeba.
The contractile vacuole does not need to work as often to remove water.
Thus, the contractile vacuoles are formed less frequently than in a normal freshwater environment.
They would not become more numerous or grow larger because the osmotic pressure inside the cell is reduced.
Answer:C. be formed less frequently