Concept:The total number of ATP produced in glycolysis means all the ATP actually synthesized during the process, not the net gain after subtracting the ATP used.
Explanation:Glycolysis splits one glucose molecule into two pyruvate molecules in the cytoplasm.
In the energy-investment phase,
2 ATP are used to phosphorylate glucose.
In the energy-payoff phase,
4 ATP are synthesized from
ADP and
Pi.
So, the total ATP produced during glycolysis is
4 ATP molecules.
The net gain is
4−2=2 ATP, but the question asks for the total produced, not the net gain.
Answer:C.
4 molecules